One cannot help but to stand in awe of the rapid progress made in the medical field. New techniques and medicines are introduced at an astonishing rate. Of course, one of the main fields of research is diagnostics. No one will argue the fact that an early diagnosis ensures a better prognosis and lower treatment costs. ELISA kits, an enzyme linked immunosorbent assay, allow doctors to diagnose a variety of conditions quickly and efficiently.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
Antibodies are protein substances that are produced by the body when it detects the presence of dangerous substances. The dangerous substances are called antigens. When antibodies are detected by means of the EIA test, it follows logically that there must be antigens too. The exact identity of the antigens can be determined by identifying the type of antibody in the sample. In this way a diagnosis can be made confidently.
The administration of the test is quick and painless. All that is required from the patient is a small blood sample. The sample is taken to a laboratory where it is added to a series of petri dishes that were prepared with different antigens. The antibodies can be identified when they react to specific antigens. The further reactions between the two agents are then studied by adding enzymes.
There are different variations of the EIA test but the more complex tests provide much more accurate results. Each type of test requires a different kit. The direct EIA test, for example is straightforward and results are available very quickly. In this case the antigens are coated with multiwall plates and the antibody can only react with the antigen once it has been conjugated with enzymes.
The indirect EIA test is much more sensitive. In this case a polystyrene plate is used to cover the antigen. The antibody can only detect the antigen during two well controlled stages. The advantage of this test is that both primary and secondary antibodies can be identified at the same time. This ability may eliminate the need for further tests, thus saving both time and money.
One of the most accurate tests is called the sandwich EIA test. In this test matched pairs of antibodies are utilized. One of the antibodies is coated with polystyrene and the other one is used as an agent for detection. One of the main advantages of this form of the test is that the concentration of analytes can be determined accurately. This can help in diagnosing latent diseases that cannot easily be done with other tests.
The EIA test is so popular with medical experts because it is versatile, quick and very accurate. The test is easy to administer and to process too. Patients benefit because their conditions can be diagnoses at an earlier stage. This makes treatment easier and it improves the prognosis.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
Antibodies are protein substances that are produced by the body when it detects the presence of dangerous substances. The dangerous substances are called antigens. When antibodies are detected by means of the EIA test, it follows logically that there must be antigens too. The exact identity of the antigens can be determined by identifying the type of antibody in the sample. In this way a diagnosis can be made confidently.
The administration of the test is quick and painless. All that is required from the patient is a small blood sample. The sample is taken to a laboratory where it is added to a series of petri dishes that were prepared with different antigens. The antibodies can be identified when they react to specific antigens. The further reactions between the two agents are then studied by adding enzymes.
There are different variations of the EIA test but the more complex tests provide much more accurate results. Each type of test requires a different kit. The direct EIA test, for example is straightforward and results are available very quickly. In this case the antigens are coated with multiwall plates and the antibody can only react with the antigen once it has been conjugated with enzymes.
The indirect EIA test is much more sensitive. In this case a polystyrene plate is used to cover the antigen. The antibody can only detect the antigen during two well controlled stages. The advantage of this test is that both primary and secondary antibodies can be identified at the same time. This ability may eliminate the need for further tests, thus saving both time and money.
One of the most accurate tests is called the sandwich EIA test. In this test matched pairs of antibodies are utilized. One of the antibodies is coated with polystyrene and the other one is used as an agent for detection. One of the main advantages of this form of the test is that the concentration of analytes can be determined accurately. This can help in diagnosing latent diseases that cannot easily be done with other tests.
The EIA test is so popular with medical experts because it is versatile, quick and very accurate. The test is easy to administer and to process too. Patients benefit because their conditions can be diagnoses at an earlier stage. This makes treatment easier and it improves the prognosis.
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